The new standard in error-free DNA
Many experiments involve tedious, error-prone, DNA cloning and assembly steps prior to even starting your assay. Error-free DNA eliminates these time consuming steps and expensive error correction procedures. Our MYdna product line offers cost-effective DNA synthesis solutions that save you both time and labor to allow more rapid testing of genes, genetic circuits, and new DNA clones. MYdna is perfect for synthetic biology applications that often require use of long DNA sequences and/or large scale assembly of DNA parts where these parts (and the final construct) need to be error-free so that testing can be done with confidence.
Double-stranded DNA Fragments, Modular Parts, Genes and Higher Order Assemblies
We combine our robust parallel oligonucleotide synthesis chemistry to a revolutionary high-throughput, automated assembly process to produce fully sequenced, error-free plasmid DNA, with 99.5% or greater of delivered DNA molecules having the correct sequence. We can deliver this DNA to you over a wide range of sizes from 20 bp up to 10 kbp or greater. With this flexible size range you can make small DNA fragments, modular parts, genes, entire genetic circuits, or higher order assemblies of your own design.
Ready, Set, Go!
Your MYdna sequence is delivered cloned into a standard plasmid vector, ready for immediate testing or assembly into larger DNA sequences using the method of your choice (Gibson, Golden Gate, etc). Our standard pBR322 plasmid can be ampicillin and/or kanamycin resistant. If desired, we can flank your custom DNA sequence with restriction sites allowing scarless enzymatic removal of your sequence.
Plasmids can be delivered as purified DNA and/or transformed E.coli cells. For purified DNA, we guarantee delivery of at least 1 µg DNA per sequence. DNA can be delivered dry or dissolved in water at a predetermined concentration. To facilitate high-throughput applications as well as storage of large sample numbers, your DNA can be sorted into 96-well microplates (or in tubes for smaller orders). *Note that DNA in solution and transformed E.coli must be shipped on dry ice.
MYdna sequences are fully compatible with Golden Gate, Gibson and PCR assembly methods, as well as our cell-free transcription-translation system MYtxtl for rapid prototyping of linear or circularized DNA constructs. Additional applications include:
- Gene synthesis
- Synthetic biology
- Modular part libraries
- Construction of genetic circuitry
- Custom sgRNA expression for CRISPR-mediated gene editing (MYcrispr)
- Homology-directed repair templates for CRISPR
- Data storage systems
- Your novel application that calls for error-free DNA!
Please note that all sequences are screened for regulated pathogens in accordance with the International Gene Synthesis Consortium (www.genesynthesisconsortium.org).
Error-free DNA Pricing
|Size||Small Order Price*||Large Order Price*||Est. Production Time*||Yield|
|20-500 bp||$99/sequence||$75/sequence||10-12 business days||≥1 µg|
|501-2000 bp||$0.18/bp||$0.15/bp||10-12 business days||≥1 µg|
|2001-5000 bp||$0.20/bp||$0.17/bp||12-15 business days||≥1 µg|
|5001-10000 bp||$0.25/bp||$0.23/bp||~20 business days||≥1 µg|
|>10 kb||Contact Us||Contact Us||Contact Us||≥1 µg|
* Prices and production times are based on a non-complex DNA sequence cloned into our standard vector, containing between 30-70% GC, without direct/inverted repeats, homopolymers of more than 20 bp, or sequences that are toxic to E.coli. Please contact us for synthesis of more complex sequences and for use of custom vectors.
Discounts for 10 or more sequences!
Small Order prices above are for orders of 1-9 DNA sequences, Large Order prices above are for an order of at least 10 sequences. Please contact us for additional cost savings on orders of 20 or more sequences, as well as for pricing on large scale DNA synthesis contracts. The more you order, the more you save!
- Email your Small or Large order Sequence Submission Form to firstname.lastname@example.org
- Once your sequences are approved, you will then be emailed an official quote
- You can then place your order with our Order Form or a PO from your institution
If you want your sequences delivered in a 96-well plate, please be sure to arrange your sequences according to their appropriate well positions in the form (Large Orders only). If these forms do not work for you, please submit a spreadsheet or tab delimited file with two columns for sequence names and sequences along with your contact information.Small Order Submission Form Large Order Submission Form
Questions? Contact us today!
We offer very competitive pricing for un-equaled error-free DNA and gene synthesis, and will work with you to determine the best product options for your research needs. Please email or call us (1-734-998-0751) with details about your needs and we can help you design customized DNA sequences that will fit your project.